7 Transfection Jobs

Date: 1 Jul 2025 Location: Bangalore, KA, IN, 560099 Custom Field 1: Dedicated Centre Job Description Designation: Associate Scientist / Senior Associate Scientist Job Location: Bangalore Department: TM-TDDT About Syngene Incorporated in 1993, Syngene International Ltd. is an innovation-focused global discovery, development and manufacturing organization providing integrated scientific services to the pharmaceutical, biotechnology, nutrition, animal health, consumer goods and specialty chemical industries around the world. Syngene’ s clientele includes world leaders such as Bristol-Myers Squibb, Baxter, Amgen, GSK, Merck KGaA and Herbalife. Its innovative culture is driven by the passion of its 4240- strong team of scientists who work with clients from around the world to solve their scientific problems, improve R&D productivity, speed up time to market and lower the cost of innovation. Job Purpose (1-2 Lines) We are seeking motivated master’s level scientists (MS, MTech, MSc, etc) to join the Translational Medicine team at the Biocon Bristol Myers Squibb Research Center in Bangalore, India. Key responsibilities of this role are to serve as an execution arm for the preclinical support of asset programs through mechanism of action-based studies, differentiation from standard of care and competitive therapeutics, combination rationale, new indications (life cycle management), and patient selection strategies. S/He will work directly with translational scientists, disease leaders within translational medicine, and will also interact with thematic research centers in early development within the company. Key Responsibilities Conduct preclinical/laboratory experiments on late stage BMS portfolio compounds to support annual goals and objectives for Translational Medicine (for internal /external, collaborations, compound specific plans etc). Responsible for delivering data in support of translational strategies including biomarkers, patient selection hypotheses, dose and schedule, combination strategies, resistance mechanisms and differentiation. Interfaces with TM scientists across departments to ensure seamless flow of information for assets which are entering the remit of translational medicine. Assists in designing experiments and delivering data for collaborative projects along with TM scientists. Maintains comprehensive records, communicates regularly and makes presentations within the department, as required. Reports experimental finding in written nonclinical pharmacology study reports, to support regulatory filings. Educational Qualification Masters with at least 2 years of relevant work experience across immunology, oncology, heme-oncology focus areas. Industry experience is a plus. Multiple positions can range upto 6 years of experience. Technical/functional Skills Cell culture, primary cell isolation, cell transfection and single cell cloning; including siRNA / CRISPR / TALEN or HDR workflows Gene expression methods including qRT-PCR and/or familiarity with RNA sequencing, epigenomic methods including methylation analysis, ChIP etc Protein analyses and immunoassays, and cell phenotyping analysis (e.g. ELISA, MSD, Luminex, TSA, CBA, flow cytometry). Design, setup and execution of autoimmune, solid tumor oncology or heme-oncology in vivo pharmacology models. Excellent verbal and written communication skills, familiarity with standard or relevant software platforms, and scientific qualities are expected. Competence in analysis and solving of problems, keeping track of overall goals. Maintain a good understanding of the methodological basis and biological significance of assays performed. Forecast potential issues regarding support of assays for current and long term projects. Devise strategic solutions to identified and potential hurdles/issues and efficiently and proactively troubleshoot technical and experimental problems. Experience 2-6 years Behavioral Skills A self-starter, who is/can become technically proficient in a diverse set of techniques and assays with a focus on quality and speed, and will meticulously follow internal SOPs Good basic lab skills required (pipetting, balances, etc.) Highly detailed and meticulous, high standards of data entering and QC Must be highly adaptive to changing timelines and goals Must be comfortable taking on complex tasks with minimal direct supervision An excellent multitasker with an ability to be level-headed in a fast-paced lab setting Team-oriented and comfortable working in a highly dynamic matrix environment across multiple locations Prior experience working with patient samples (i.e. FFPE tissue, Fresh Frozen tissue, blood) or working in a CLIA/GCLP/clinical environment is a plus Must be comfortable and able to work with animal (rodent) or human derived samples Equal Opportunity Employer It is the policy of Syngene to provide equal employment opportunity (EEO) to all persons regardless of age, color, national origin, citizenship status, physical or mental disability, race, religion, creed, gender, sex, sexual orientation, gender identity and/or expression, genetic information, marital status, status with regard to public assistance, veteran status, or any other characteristic protected by applicable legislation or local law. In addition, Syngene will provide reasonable accommodations for qualified individuals with disabilities.

Date: 1 Jul 2025 Location: Bangalore, KA, IN, 560099 Custom Field 1: Dedicated Centre Job Description Designation: Associate Scientist / Senior Associate Scientist Job Location: Bangalore Department: TM-TDDT About Syngene Incorporated in 1993, Syngene International Ltd. is an innovation-focused global discovery, development and manufacturing organization providing integrated scientific services to the pharmaceutical, biotechnology, nutrition, animal health, consumer goods and specialty chemical industries around the world. Syngene’ s clientele includes world leaders such as Bristol-Myers Squibb, Baxter, Amgen, GSK, Merck KGaA and Herbalife. Its innovative culture is driven by the passion of its 4240- strong team of scientists who work with clients from around the world to solve their scientific problems, improve R&D productivity, speed up time to market and lower the cost of innovation. Job Purpose (1-2 Lines) We are seeking motivated master’s level scientists (MS, MTech, MSc, etc) to join the Translational Medicine team at the Biocon Bristol Myers Squibb Research Center in Bangalore, India. Key responsibilities of this role are to serve as an execution arm for the preclinical support of asset programs through mechanism of action-based studies, differentiation from standard of care and competitive therapeutics, combination rationale, new indications (life cycle management), and patient selection strategies. S/He will work directly with translational scientists, disease leaders within translational medicine, and will also interact with thematic research centers in early development within the company. Key Responsibilities Conduct preclinical/laboratory experiments on late stage BMS portfolio compounds to support annual goals and objectives for Translational Medicine (for internal /external, collaborations, compound specific plans etc). Responsible for delivering data in support of translational strategies including biomarkers, patient selection hypotheses, dose and schedule, combination strategies, resistance mechanisms and differentiation. Interfaces with TM scientists across departments to ensure seamless flow of information for assets which are entering the remit of translational medicine. Assists in designing experiments and delivering data for collaborative projects along with TM scientists. Maintains comprehensive records, communicates regularly and makes presentations within the department, as required. Reports experimental finding in written nonclinical pharmacology study reports, to support regulatory filings. Educational Qualification Masters with at least 2 years of relevant work experience across immunology, oncology, heme-oncology focus areas. Industry experience is a plus. Multiple positions can range upto 6 years of experience. Technical/functional Skills Cell culture, primary cell isolation, cell transfection and single cell cloning; including siRNA / CRISPR / TALEN or HDR workflows Gene expression methods including qRT-PCR and/or familiarity with RNA sequencing, epigenomic methods including methylation analysis, ChIP etc Protein analyses and immunoassays, and cell phenotyping analysis (e.g. ELISA, MSD, Luminex, TSA, CBA, flow cytometry). Design, setup and execution of autoimmune, solid tumor oncology or heme-oncology in vivo pharmacology models. Excellent verbal and written communication skills, familiarity with standard or relevant software platforms, and scientific qualities are expected. Competence in analysis and solving of problems, keeping track of overall goals. Maintain a good understanding of the methodological basis and biological significance of assays performed. Forecast potential issues regarding support of assays for current and long term projects. Devise strategic solutions to identified and potential hurdles/issues and efficiently and proactively troubleshoot technical and experimental problems. Experience 2-6 years Behavioral Skills A self-starter, who is/can become technically proficient in a diverse set of techniques and assays with a focus on quality and speed, and will meticulously follow internal SOPs Good basic lab skills required (pipetting, balances, etc.) Highly detailed and meticulous, high standards of data entering and QC Must be highly adaptive to changing timelines and goals Must be comfortable taking on complex tasks with minimal direct supervision An excellent multitasker with an ability to be level-headed in a fast-paced lab setting Team-oriented and comfortable working in a highly dynamic matrix environment across multiple locations Prior experience working with patient samples (i.e. FFPE tissue, Fresh Frozen tissue, blood) or working in a CLIA/GCLP/clinical environment is a plus Must be comfortable and able to work with animal (rodent) or human derived samples Equal Opportunity Employer It is the policy of Syngene to provide equal employment opportunity (EEO) to all persons regardless of age, color, national origin, citizenship status, physical or mental disability, race, religion, creed, gender, sex, sexual orientation, gender identity and/or expression, genetic information, marital status, status with regard to public assistance, veteran status, or any other characteristic protected by applicable legislation or local law. In addition, Syngene will provide reasonable accommodations for qualified individuals with disabilities.

Summary of Job Hands-on experience on Biochemical and Cell-based assays. Key Responsibilities Cell based assays: Candidate with good knowledge on cell culturing techniques & cell-based assays. Ex: Cell viability assay, Transfection, High-throughput Screening, Western Blotting, multiplex ELISA, Immunofluorescence assays, and Cell migration assays, flowcytometry etc. Experience on PBMC isolation, T cell purification/activation/differentiation assays. Biochemical assays: Hands on experience of conducting biochemical assays (HTRF, ADP-Glo, Alpha LISA) Should have hands on experience on other cellular assays- Reporter assays, Cytotoxicity assays, gene expression studies etc. Should independently work and analyze the data. Well versed with the data analysis software, Excel, Prism-GraphPad & FlowJo. Key Competencies Technical: Multi-channel pipettes, Multimode plate readers, SDS-PAGE, western blot, operation of centrifuge, ELISA etc. Functional Good hands-on experience on Biochemical, and Cell based assays. Behavioral: Team player, ability to work with multidisciplinary teams, Strong analytical skills and ability to work under stringent timelines Good communication skills

Molecular Biology activities like plasmid and genomic DNA preparation, PCR amplification, restriction digestion, molecular cloning, sequence confirmation, involved in vector construction for stable and transient gene expression. Routine cell bank preparation of microbial and mammalian cells. Analytical skills related to protein estimation and characterization like, ELISA, SDS PAGE/Western Blot, Octet and HPLC. Standard cell culture activity such as vial thaw, subculturing, transfection, single cell cloning, fed batch, monoclonality assurance. Experience in bacterial and yeast expression systems preferred. Routine documentation in electronic notebook. Preparation of SOP/GM and development reports. Industrial experience of 4-6 years in cell line development.

Profile BRIC-inStem invites applications (in online mode) from eligible candidates for a contractual Scientific position in the laboratory of Dr. Bhavana Muralidharan, inStem Bangalore. They are looking for dedicated professionals who are result-oriented, can assimilate, analyze and communicate data effectively, and are excellent team players capable of working in a fast-paced, multi-disciplinary environment. This position is available in the lab of Dr. Bhavana Muralidharan, who studies brain development in health and disease. One of the candidates will work with Dr. Kruttika Phalnikar, a DBT Wellcome Trust Early Career fellow. The ideal candidate should be proficient in working with stem cell and mammalian cell culture, mouse brain, and basic and advanced molecular biology techniques such as cloning, transfection, and epigenetic analysis. Additional skills include confocal/fluorescent microscopy. The candidate should possess excellent written and oral communication skills, strong organizational and planning abilities, and a demonstrated record of scientific productivity. The ability to work independently and collaboratively within a team is essential. Duration Contractual appointment for 11 months, renewable based on requirement and review of progress and requirement of the Grant. Money Rs. 25,000 pm 27 PERCENT HRA. Qualification Masters degree in Science from a recognised university. To Apply Interested candidates may log into the BRIC-inStem website Careers Open positions (https://www.instem.res.in/jobp) Mode Of Applications Only online applications will be accepted. Applications received through any other mode shall stand rejected automatically. Applicants should submit the following information as a single PDF file: 2page Curriculum vitae with current address, phone (mobile) number, and email address. 1page Motivation letter explaining past research experiences, career goals, and motivation to join our laboratory and research programs. Please include the advertisement number in the letter and upload the same as one document with a CV. Contact information of at least two referees (will be contacted only if needed) For more details click here. Show more Show less

Molecular Biology activities like plasmid and genomic DNA preparation, PCR amplification, restriction digestion, molecular cloning, sequence confirmation, involved in vector construction for stable and transient gene expression. Routine cell bank preparation of microbial and mammalian cells. Analytical skills related to protein estimation and characterization like, ELISA, SDS PAGE/Western Blot, Octet and HPLC. Standard cell culture activity such as vial thaw, subculturing, transfection, single cell cloning, fed batch, monoclonality assurance. Experience in bacterial and yeast expression systems preferred. Routine documentation in electronic notebook. Preparation of SOP/GM and development reports. Industrial experience of 4-6 years in cell line development. Show more Show less

Job Title: Research Scientist – Molecular Biology Location : Varanasi, Uttar Pradesh (Inside BHU Campus, On-Site) Job Type : Full-time Joining Type: Immediate About Us: Technoculture Research Pvt. Ltd. is a leading research and development company focused on innovative solutions in biotechnology, engineering, and software development. We specialise in bio-instrumentation research, pharmacogenomics-based drug testing, and precise diagnosis of infectious diseases. Our technology-driven approach ensures cutting-edge advancements in healthcare and enterprise solutions. Job Description: We are seeking a highly skilled and motivated Research Scientist with a Ph.D. in Molecular Biology and a solid background in cell culture techniques. The ideal candidate should have extensive hands-on experience in molecular biology methods and strong proficiency in designing and conducting independent experimental research. This role offers a unique opportunity to work in a collaborative and innovation-driven environment within the prestigious BHU campus. Responsibilities: Design and execute experiments in molecular biology with a focus on gene expression, genetic modification, and cell signalling pathways. Should be able to design vectors and primers for downstream processes. Perform advanced cell culture techniques including maintenance, transfection, and viability assays in mammalian cell lines. Apply core molecular biology techniques such as DNA/RNA isolation, PCR, qPCR, Western blotting, and cloning. Should be able to create stable cell lines using transfection and transduction. Analyse experimental data, troubleshoot protocols, and optimise methods to improve outcomes. Maintain accurate documentation of experiments, results, and methodologies. Present research findings to internal teams and contribute to publication and patent efforts. Adhere to safety protocols, standard operating procedures, and quality control standards. Qualifications: Ph.D. in Molecular Biology or a closely related discipline. Strong expertise in cell culture techniques and mammalian cell handling. Demonstrated experience in molecular biology techniques including PCR, qPCR, Western blotting, plasmid preparation, cloning, transfection, transduction, and electroporation. Ability to work independently on experimental design and data interpretation. Preferred Qualifications: Postdoctoral or industry research experience in cell and molecular biology. Exposure to RNA-based technologies or translational biology. Familiarity with microscopy and flow cytometry is an advantage. Basic understanding of bioinformatics tools for gene and protein analysis. Skills: Excellent analytical, problem-solving, and troubleshooting skills. Strong documentation and communication abilities (written and verbal). Well-organised and detail-oriented with good time management. Ability to work in a fast-paced, team-oriented environment. Personal Attributes: Passionate about scientific innovation and research excellence. Self-driven, proactive, and committed to continuous learning. Capable of taking initiative and working with minimal supervision. Collaborative mindset with the ability to work across diverse scientific disciplines. Show more Show less